Figure 1. Shuttle vector with GFP expression under Tet control.

The E. coli cloning vector pASK-IBA33plus, with GFP inserted under Tet control, was ligated to the wild-type plasmid from C. trachomatis strain L2 using PCR-generated restriction endonuclease sites (see Materials and Methods for details). A variation of this shuttle vector was later constructed with a gene encoding mKate2 inserted at the indicated KasI site.