Figure 4. Detection of constitutive mKate2 and inducible GFP.

L929 cell cultures infected with pASK-GFP/mKate2-L2 transformed C. trachomatis were induced at 24 hpi and images were then taken at indicated time points. Representative images from an uninduced sample and a sample induced with 10 ng/mL ATc at 24 hpi are shown in (A). Representative images of a sample induced with 10 ng/mL ATc at 16 hpi (from a separate experiment) are shown in (B). White arrows highlight the relatively few C. trachomatis cells within the inclusion that did not express detectable GFP at 19 hpi and 20 hpi.