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Plasmids harbouring the RPO30 gene of GTPV, SPPV and LSDV were used as templates. The melting regions of the PCR products duplexes are located between the two colored thick vertical lines; the blue lines are flanking the melting region of snapback hairpins (50–60°C) while the orange lines are flanking the full-length amplicons (70–76°C).
