Table 1. Sequences of the snapback and reverse primers.
Primers | Sequences |
Snapback | 5′-ggTGTAGTACGTATAAGATTATCGTATAGAAACAAGCCTTTA-3′ |
Reverse | 5′-AATTTCTTTCTCTGTTCCATTTG-3′ |
The snapback tail of 16 bases is shown as underlined; 2 nucleotides mismatch at the 5′end are indicated as lowercase; and mismatch nucleotide as underlined bold (A).