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A: Perfectly positioned if the distance between the TR and the start codon is ±40, ±60 or ±100 nt. B: The TR is intragenic. C: If the TR was located between 500 nt up-stream of the first codon and distance A, it was considered as possibly related to the gene. D: If the TR was located more than 500 nt up-stream any annotated ORF.
