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. 2013 Oct 7;8(10):e76184. doi: 10.1371/journal.pone.0076184

Table 2. List of new genes and genes to be re-annotated.

Position Blast result Conclusion
CH991763∶266007-267494_R P23-like domain, similar to GL50581_3538 figGiardiaintestinalis ATCC 50581] new gene, similar to other assemblage
CH991763∶264707-265966_R IFT complex B, GL p15, re-annotate GL50803_40995 gene to be re-annotated
CH991817∶18,017-18,463 Hypothetical protein, re-annotate GL50803_25713 gene to be re-annotated
CH991814∶252,697-256,896 Hypothetical protein GL P15/kinase protein new gene/conserved domain
CH991803∶2702-4543 VSP, similar to GL50803_114065 gene repeat/conserved domain, FU-like and VSP domains,re-annotate GL50803_102540
CH991798∶30,210-30,809 ribosomal protein S11 re-annotate (GL50803_14827) 199AA instead of 154, similarto other assemblage
CH991782∶26,231-28,138_R VSP, conserved domain 635 instead of 195 AA, original gene GL50803-101380,gene to be re-annotated
CH991779∶250938-252914 -R VSP, conserved domain gene repeat
CH991779∶569,674-571,152 VSP, conserved domain new gene
CH991779∶1,223,042-1,223,698 Hypothetical protein, GL P15 new gene, similar to other assemblage
CH991779∶1,425,747-1,427,552 VSP, conserved domain gene to be re-annotated, re-annotate GL50803_40630
CH991785∶11,532-11,867_R SORL conserved domain, hypothetical protein GL P15 new gene
CH991776∶59,721-59,930 ribosomal S30 conserved domain new gene
CH991771∶171,536-171,874_R(112AA) similar to hypothetical protein GL50803_32738 gene repeat
CH991769∶78,752-81,292 hypothetical protein, two conserved domains new gene, similar to other assemblage GL P15
CH991769∶412,442-413,248 similar to reverse transcriptase gene repeat
CH991769∶624,472-624,627 50S ribosomal protein L39e new gene
CH991769∶770,102-771,508 hypothetical protein similar gene/gene repeat, similar to other assemblageGL P15/hypothetical GL50803_17273
CH991768∶744,494-745,936 Hypothetical protein new gene similar to similar to other assemblageGL P15
CH991768∶1,281,692-1,282,111-R GL P15, Ribosomal protein S19e domain conserved new gene-reverse strand, CH991768∶1,281,692-1,282,111-R
CH991764∶147,549-148,025_R partially similar to hypothetical protein GL50803_20672 new gene
CH991764∶148,024-149,931 VSP conserved domain, new gene
,CH991762∶115,657-116,463 ANK conserved domain new gene/gene repeat, similar to GL P15, Ser/Thrprotein kinase
CH991761∶101,085-102,872 VSP conserved domain, similar GL50803_116477 gene repeat, re-annotate GL50803_135831
CH991761∶113,307-113,858 VSP conserved domain gene repeat
CH991761∶113,432-113,770_R similar to hypothetical protein GL50803_105806 gene repeat
CH991761∶295,809-301,103 Hypothetical protein, GL P15, conserved domain WD40 new gene, partially similar to Hypothetical protein(GL50803_113673)
CH991763∶4,689-7,532_R conserved domain, Ankyrin-like and protein kinase gene repeat, similar to GL50803_113094
CH991763∶689121-689569 partially similar to GL50803_101496 partial gene repeat
CH991763∶688,749-688,942_R partially similar to GL50803_137676, kinase partial gene repeat
CH991767∶1667698-1667877 conserved domain, Ferredoxin Fd1, Fd2 partial gene repeat
CH991761∶301,967-303,142 conserved domain, NEK, kinase-like new gene/gene repeat
CH991761∶302,965-305,298 ANK conserved domain, similar to kinase new gene
CH991763∶1395469-1397541_R VSP conserved domain, similar to High cysteine membraneprotein Group 1 (GL50803_91707) new gene
CH991767∶885323-886138 VSP domain, similar to P15 partial gene repeat
CH991767∶1127974-1130037_R VSP domain, similar to P15 new gene
CH991767∶1130397-1135277 hypothetical protein, similar to P15 new gene
CH991767∶1135382-1140265 conserved domains, chromosome segregation protein SMC, similar to Axoneme-associated protein GASP-180 gene repeat
CH991767∶1140362-1145860 re-annotate GL50803_32999 to be similar to P15 (GLP15_1881) many conserved domains
CH991767∶1146036-1147784 conserved ANK domain, Coiled-coil protein [Giardia intestinalis ATCC 50581], Hypothetical protein GL50803_41212 new gene
CH991767∶1696020-1696778 conserved ANK domain and zinc finger, similar to GL50803_113284 hypothetical protein and Protein 21.1 P15 gene repeat
CH991793∶23497-23754 ORF with low similarity new gene, well expressed in RNA-seq
CH991763∶1,306,665-1,307,180 ORF with low similarity new gene, expressed in RNA-seq
CH991776∶157233-158693 conserved ANK and kinase domains, partially similar to?NEK (GL50803_93221) new gene/gene repeat
CH991762∶387,382-387,645 partially similar to hypothetical protein GL50803_38965 partial gene repeat
CH991763∶692,405-692,956 partially similar to GL50803_31921, new gene
CH991763∶692571-693002 partially similar to hypothetical protein GL50803_5692 new gene
CH991767∶340089-340346 mostly retrotransposon gene repeat/new gene
CH991767∶436,937-437,701_R similar to VSP,(GL50803_111732) gene repeat
CH991767∶435261-437231 similar to high cysteine membrane protein EGF-like (GL50803_114626) gene repeat
CH991782∶818,861-820,612 similar to P15 and 50581 strains re-annotate GL50803_40224
CH991761∶20575-22203 similar to P15 and 50581 strains re-annotate GL50803_96616
CH991767∶1,732,248-1,734,773 similar to P15 and 50581 strains re-annotate GL50803_39210
CH991779∶262026-264188 similar to P15 and 50581 strains re-annotate GL50803_35276
CH991776∶21991-23994 similar to P15 and 50581 strains re-annotate GL50803_34684
CH991779∶1223042-1223698 new hypothetical protein, conserved among 3 assemblages new gene, expressed in in RNA-seq
CH991769∶937870-939393 new hypothetical protein, conserved among 3 assemblages, conserved Ribophorin I domain new gene, expressed in in RNA-seq
CH991814∶199061-199591 similar to GL50803_114246, GTP-binding protein, putative partial gene repeat
CH991779∶1,155,366-1,156,079_R similar to Rossmann-fold protein [Giardia lamblia P15],conserved putative domain new gene
CH991769∶2,224-3,885_R similar to hypothetical protein GLP15_2551 new gene
CH991769∶953,848-955,386_R similar to P15 hypothetical protein re-annotate GL50803_7035
CH991763∶1385753-1387552_R similar to hypothetical protein in P15 and 50581 strains new gene
CH991779∶681,867-683,660_R similar to P15 and 50581 strains re-annotate GL50803_2822
CH991776∶278,076-279,610_R PTZ00382 conserved domain re-annotate GL50803_97233, well expressed in RNA-seq
CH991769∶77,021-78,700 new hypothetical protein, similar to P15 and 50581 new gene
CH991769∶56,305-56,718_R new hypothetical protein, similar to P15 and 50581 new gene
CH991767∶707,305-707,724_R new hypothetical protein, similar to hypothetical protein GLP15_3559 new gene
CH991769∶334626-334943_R Gene repeat, Giardia lamblia ATCC 50803 Pam18p (GL50803_300001) gene repeat
CH991767∶707,305.707,724_R similar to hypothetical protein GLP15_3559 new gene
CH991776∶310,552-313,605_R new hypothetical protein, similar to P15 and 50581 new gene
CH991814∶296,825-303,154_R new hypothetical protein, similar to P15 and 50581 new gene
CH991769∶494114-494923 new hypothetical protein, similar to P15 and 50581 new gene
CH991814∶275,394-281,945 similar to Kinase [Giardia lamblia P15], multiple conserved domain new gene
CH991779∶986627-987556_R similar to Kinase GL50803_101307 and GL50803_86934 gene repeat
CH991793∶39014-40039_R new hypothetical protein, similar to P15 and 50581 new gene
CH991763∶195,962-199,271_R similar to P15 and 50581 strains re-annotate GL50803_32861
CH991780∶48,596-52,006_R similar to P15 and 50581 strains re-annotate GL50803_41369
CH991763∶487,379-487,747 new hypothetical protein, similar to P15 and 50581 new gene
CH991767∶1652396-1654378 similar to P15 and 50581 strains re-annotate GL50803_41311
CH991771∶3728-4342 similar to GLP15_4099 re-annotate GL50803_40244
CH991767∶473391-475715_R similar toGLP15_5080 and GL50581_209 re-annotate GL50803_36426
CH991769∶546414-550226_R similar to GLP15_5033 and GL50581_4447 re-annotate GL50803_103205
CH991776∶41095-43620_R similar to GLP15_3901 re-annotate GL50803_39904
CH991768∶596,759-597,880_R similar to P15 and 50581 strains re-annotate GL50803_30448