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| Mutant | TM [°C] |
| Wild-type HypT | 59.0 |
| HypT5C→S | 50.5 |
| HypTC4S | 60.8 |
| HypTC25S | 58.8 |
| HypTC150S | 54.3 |
| HypTC178S | 59.7 |
| HypTC242S | 57.9 |
| HypTquadrupleC4 | 50.0 |
| HypTquadrupleC25 | 51.0 |
| HypTquadrupleC150 | 57.3 |
| HypTquadrupleC178 | 50.2 |
| HypTquadrupleC242 | 52.5 |
