Figure 7. Effect of NMK-TD-100 on tubulin polymerization in cell-free system.

(A) Tubulin was polymerized in the presence of various concentration of NMK-TD-100 (0-30 µM) and the kinetics of polymerization was monitored by increase using light scattering at 350 nm. (B) Transmission electron microscopic studies of products of tubulin polymerization in the absence and presence of 25 µM of NMK-TD-100. Scale bar is 500 nm. (C) Effect of NMK-TD-100 on depolymerization of preformed microtubule as monitored by decrease in light scattering at 350 nm. The end point absorbance at 350 nm was plotted against the concentration of NMK-TD-100. Data are representative of average of three similar experiments with SD.