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. 2013 Oct 7;8(10):e76914. doi: 10.1371/journal.pone.0076914

Figure 7. Complementation of appressorium formation, penetration and pathogenicity of the Δmet13 mutant by adding exogenous methionine.

Figure 7

(A) Appressoria of the wild-type strain Guy11, K56 (Δmet13) and K12-7(Δmet12) were induced on hydrophobic coverslips (HC) and onion epidermis (OE) surfaces at 25°C in darkness for 24 h. Bar represents 10 μm for top panels and 20 μm for bottom panels. (B) Rice infection assays. Conidial suspension of 1 × 105 conidia ml-1 were spray-inoculated onto rice seedlings. H2O was used as the negative control. Photographs were taken at 5 days after inoculation. For both A and B, conidia of Guy11, K56, C3 (Δmet13MET13), K12-7 and DK7 (Δmet13Δmet12) were harvested from the CM cultures with 2 mM methionine and then diluted in 1 mM methionine solution.