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. Author manuscript; available in PMC: 2013 Oct 8.
Published in final edited form as: Virology. 2009 Sep 3;393(1):1–6. doi: 10.1016/j.virol.2009.07.036

Fig. 2. Serologic analysis of BPV1 and BPV2 by VLP ELISA and virion neutralization assays.

Fig. 2

BPV1 VLP (a) or BPV2 VLP (b) were analyzed under non-denaturing (native) conditions using antisera raised to BPV1-L1 and BPV2-L1 VLPs, and mAb directed to conformational BPV1 epitopes. VLPs were incubated with 10-fold serial dilutions (10−2 to 10−5) of antibodies. Data are expressed as mean OD±SD of triplicate wells. Neutralization capacity of antibodies at final dilution of 1:100 was determined by C127 focus-forming neutralization assays using BPV1 (a) or BPV2 (b) native virions. +, Neutralizing, −, non-neutralizing; n.d., not determined.