Figure 6.

Noisy expressions of collagenase 1 and cox-2 mRNAs, in spite of relatively constant levels of c-fos/c-jun heterodimers. (A) Triplex detection of cox-2 and collagenase 1 mRNAs and c-fos/c-jun heterodimers 6 h after serum induction. The three left-hand panels are raw merged z-stacks showing the indicated targets, and the right-hand panel shows the identified molecules overlaid on an image of cells stained for their nuclei. (B) Time course of the appearance of nuclear c-fos/c-jun heterodimers and two downstream mRNAs after serum induction in the whole cells. (C) Distributions of nuclear c-fos/c-jun heterodimers and whole cell collagenase 1 and cox-2 mRNAs in 75 single cells 6 h after serum induction. Red dots depict the data obtained from the cells shown in (A). Correlation between collagenase 1 and cox-2 mRNAs is presented in Supplementary Figure S3. Source data for this figure is available on the online supplementary information page.

Source Data for Figure 6B-C ^{(50.9KB, xlsx)}