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. Author manuscript; available in PMC: 2014 Oct 3.
Published in final edited form as: Neuron. 2013 Oct 2;80(1):64–71. doi: 10.1016/j.neuron.2013.07.014

Figure 3. Ephrin-A2 Colocalizes with Astrocytic Glutamate Transporters in the Mouse Cortex.

Figure 3

(A, B) Representative image volumes of ephrin-A2 and ephrin-A3 immunofluorescence staining in superficial layer I of one-month-old mouse cortex. Each image is a maxium projection of 5 serial sections. Scale bar, 10 µm. Data are presented as mean ± SEM. *P<0.05.

(C) Quantification of puncta density shows that ephrin-A2 is approximately 3 times enriched, compared to ephrin-A3 in the cortex.

(D) Maximum projection of 36 serial sections of ephrin-A2 immunofluorescence (red) with protein markers of presynaptic SV2 (orange), postsynaptic PSD95 (white), astrocytic GLT-1 (magenta) and dendritic segment labeled with YFP (green). Scale bar, 1 µm.

(E) Images of a spine from the boxed region in (D) at higher magnification with various protein markers. Scale bar, 500 nm.

(F) Average numbers of ephrin-A2 puncta within 100 nm from the centers of different neuronal and astrocytic constituents.

See also Figure S4.