FIGURE 10.

Interactions of the wild-type, mutated, and truncated NTRC in Y2H tests. Yeast CY306 cells were transformed with NTRC, truncated NTRd or TRXd, and with mutated NTRC constructs with both or the second Cys of the redox active site of NTRd (CAIS construct), or TRXd (SGPS and CGPS constructs, respectively) replaced by Ser. The cells were grown into stationary phase and adjusted to an OD₆₀₀ of 0.05, 0.005 and 0.0005 before spotting onto plates containing histidine (+His) or without histidine (-His). Yeast cells were photographed after four days growth at 28°C. VPg + elF4G and empty vector was used as positive and negative controls, respectively (see Materials and Methods).