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. 2013 Oct 8;4:389. doi: 10.3389/fpls.2013.00389

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Copyright © Toivola, Nikkanen, Dahlström, Salminen, Lepistö, Vignols and Rintamäki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heat-induced monomerization of NTRC homodimers. Proteins were extracted from leaves in the presence (+) or absence (-) of DTT and solubilized in SDS-buffer with (+) or without (-) mercaptoethanol (ME; Laemmli, 1970) on ice (-) or heated at 100°C for 1 min (+). Amounts of leaf extracts (μg soluble protein, upper panel) were loaded on SDS-gel and the proteins were separated in SDS-PAGE before Western blotting with anti-NTRC antibody or anti-NTRB antibody. D-NTRC and M-NTRC, dimeric and monomeric NTRC proteins, respectively. NTRd?, truncated NTRd with anomalous movement in SDS-gel. For other abbreviations, see the legend for Figure 3.