Table 2.
Leaf number, chlorophyll content and dry weight of transgenic rosettes overexpressing wild-type, mutated NTRC, or truncated NTRC domains.
| Line | Chl a + b (μg cm-2 ± SE) (% of WT) | Chl a/b ± SE | Dry weight (mg ± SE) (% of WT) | Leaf number ± SE |
|---|---|---|---|---|
| SD 130 μmol m-2 s-1 | ||||
| WT | 26.3 ± 1.2 (100) | 3.13 ± 0.08 | 197 ± 18 (100) | 38 ± 1 |
| OE-NTRC | 19.9 ± 1.8 (76)* | 3.13 ± 0.08 | 280 ± 46 (142) | 39 ± 1 |
| ntrc | 3.0 ± 0.5 (11)*** | 2.61 ± 0.09** | 24 ± 5 (12)*** | 22 ± 1*** |
| OE-SGPS | ||||
| Young | 6.0 ± 0.2 (23)*** | 2.78 ± 0* | 108 ± 12 (55)** | 37 ± 1 |
| Mature | 16.4 ± 1.6 (63)** | 3.09 ± 0.04 | ||
| OE-SAIS | ||||
| Young | 8.3 ± 0.6 (32)*** | 2.98 ± 0.07 | 102 ± 7 (52)** | 34 ± 1* |
| Mature | 14.1 ± 0.7 (54)*** | 3.18 ± 0.07 | ||
| OE-NTRd | ||||
| Young | 5.9 ± 0.5 (22)*** | 2.85 ± 0.10 | 51 ± 3 (26)*** | 30 ± 1*** |
| Mature | 10.9 ± 0.7 (41)*** | 3.13 ± 0.04 | ||
| OE-TRXd | ND | ND | 56 ± 2 (28)*** | 28 ± 1*** |
| SD 600 μmol m-2s-1 | ||||
| WT | 13.5 ± 0.4 (100) | 3.90 ± 0.04 | 334 ± 13 (100) | ND |
| OE-NTRC | 14.9 ± 0.2 (110)* | 3.90 ± 0.08 | 877 ± 57 (263)*** | ND |
| ntrc | 7.5 ± 0.5 (55)*** | 3.35 ± 0.09*** | 33 ± 4 (10)*** | ND |
| OE-SGPS | ||||
| Young | 7.9 ± 0.5 (58)*** | 3.93 ± 0.11 | 176 ± 16 (53)** | ND |
| Mature | 14.5 ± 1.1 (107) | 4.01 ± 0.10 | ||
| OE-SAIS | ||||
| Young | 8.6 ± 0.8 (63)*** | 3.75 ± 0.08 | 354 ± 47 (106) | ND |
| Mature | 14.9 ± 0.7 (110) | 4.09 ± 0.05* | ||
| OE-NTRd | ||||
| Young | 9.0 ± 1.0 (67)** | 3.93 ± 0.06 | 112 ± 18 (34)*** | ND |
| Mature | 16.7 ± 1.1 (124)* | 4.02 ± 0.06* | ||
| OE-TRXd | ||||
| Young | 7.7 ± 0.4 (57)*** | 3.86 ± 0.10 | 45 ± 5 (13)*** | ND |
| Mature | 10.4 ± 0.7 (77)** | 4.08 ± 0.08 |
Plants were grown under short day conditions (SD) for 7 weeks in the light intensity indicated in the table. Leaf number, chlorophyll content (Chl a+b), chlorophyll a/b ratio (Chl a/b) and rosette dry weight were measured as described in Section “Materials and Methods.” Each value is the mean ± SE of four to five independent determinations. Chlorophyll content of transgenic lines overexpressing mutated NTRC was measured from both young yellowish and green (mature) leaves. ND, not determined. *P, **P, ***P < 0.05, 0.01, 0.001 (student’s t-test), respectively, compared with WT plants. For the other abbreviations, see the legend for Figure 3.