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. 2013 May 24;20(11):1465–1474. doi: 10.1038/cdd.2013.52

Figure 1.

Figure 1

p53 KO MEF cells, but not WT or Bax Bak DKO, survive to DNA damage induced by H2O2. (a) To assess DNA damage extent, cells were exposed to 1 mM H2O2 for 30 min and immunostained using anti-phospho Histone H2AX antibody (red). Nuclei were stained using DAPI (blue) and observed under a confocal microscope. Similar DNA damage extent was observed after treatment in all three cell lines. (b) WT MEF, Bax Bak DKO and p53 KO were treated for 24 h with 1 mM H2O2. Cells were stained with fluorescent conjugates of annexin-V and propidium iodide (PI) and analyzed by FACS. Viable cells are annexin-V negative and PI negative, and cell death is expressed as 100%-viable cells. Values indicate mean values±S.E.M. All experiments were performed independently at least three times (N≥3). **P<0.01 (compared with WT MEF H2O2-treated). (c) Cell death is expressed as 100% - viable cells. Representative FACS plots are shown