Figure 1.
Nucleolar activity and integrity are decreased in R6/2 models of HD. (a) Visualization of 47S pre-rRNA by in situ hybridization on striatal sections of 7-week-old control (upper panel) and R6/2 mice (lower panel). (b) RT-qPCR analysis of 18S rRNA expression in striata from R6/2 mice (n=4) and controls (n=8) at 7 and 13 weeks. Values represent means±S.E.M. (c and d) Quantitative analysis of striatal cells with decreased nucleolar NPM/B23 (% of cells per mm2) in control and 7-week- old R6/2 mice. Values represent means±S.E.M. (n=3–4). Scale bars: 60 μm