Figure 3.

Protein levels of ERα and ERβ are not altered by ovarian hormone loss or in vivo E₂ treatment. A, Representative Western blots of ERα, ERβ, and GAPDH. The antibodies detected bands of ∼66 kDa for ERα and ∼55 kDa for ERβ. The numbers in the parentheses indicate the numbers of hippocampi (one per rat) included for each experimental group; each sample was run in duplicate and the signal was averaged. These averaged values for individual hippocampus were then used to determine the plotted average values ± SEM for each treatment group of rats. B, Summary bar graphs of the relative levels of protein expression for ERα (left) and ERβ (right), normalized to GAPDH.