Figure 4.

Modulation of intrinsic Kv1.3 channel properties by sevoflurane. A, Kv1.3 currents in one representative L929 cell activated with 10 mV increments and interpulse intervals of 30 s in control conditions and in the presence of sevoflurane. B, Kv1.3 current evoked by depolarizing voltage pulse to −30 mV is potentiated by sevoflurane and blocked by ShK and ShK-186, specific Kv1.3 channel blockers. C, The potentiation of Kv1.3 current at −30 mV after preexposure to sevoflurane for 10 s, 30 s, and 5 min. D, Sevoflurane modulates Kv1.3 channels in closed configuration (n = 3 experiments). The control current (black) was recorded before exposure to sevoflurane (red). The second current trace (depolarization to either +30 or −30 mV) was evoked 45 s after the addition of sevoflurane without pulsing (channels are closed), and the third current trace was evoked 45 s later. The second trace at −30 mV is superimposed over the third trace (gray over red). The data are presented as mean ± SEM; n = 3 cells for each data point; paired t test. n/s, Not significant.