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. 2013 Sep 15;27(18):1949–1958. doi: 10.1101/gad.220194.113

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© 2013, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 4. — Development of mGS cells after Sox2 or Oct4 overexpression (OE). (A) Real-time PCR analysis of Dmrt1 target genes in p53 knockout (KO) GS cells after Dmrt1 knockdown (KD) (n = 3). pLKO.1 EGFP was used as a control. (B) Suppression of mGS cell development by Sox2 knockdown 28 d after Dmrt1 knockdown (n = 13). pLKO.1 EGFP was used as a control. (C) Real-time PCR analysis of Dnmt1, Dmrt1, Sox2, and Oct1/4 expression in p53 knockout GS cells 14 d after Dmrt1 knockdown (n = 4). pSicoR was used as a control. For concurrent Dmrt1 overexpression, CSII-EF1α-IRES2-Venus was a control. (D) Real-time PCR analysis of Oct4 expression in p53 knockout GS cells 14 d after Sox2 overexpression (n = 3). CSII-EF1α-IRES2-hKO1 was used as a control. (E) Reduced Sox2-mGS cell development 28 d after Oct4 knockdown (n = 9).