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. 2013 Sep 15;27(18):1974–1985. doi: 10.1101/gad.226613.113

Figure 2.

Figure 2.

IDH2 mutations result in AML. (A) Blood smear of recipient mice transplanted with Flt3-ITD or NrasG12D HSPCs transduced with IDH2 wild type (WT) or mutants (IDH2R140Q and IDH2R172K). (B) H&E staining of BM sections (400×) of recipient mice transplanted with IDH2 wild-type- or mutant-expressing cells. (C) Representative flow plots of BM cells from recipient mice transplanted with Flt3-ITD or NrasG12D HSPCs transduced with IDH2 wild type, or mutants (IDH2R140Q and IDH2R172K). (D) Representative flow plots of splenocytes stained with Mac-1 and c-kit of recipients transplanted with Flt3-ITD cells transduced with empty vector, IDH2 wild type, or mutants (IDH2R140Q and IDH2R172K). (E) Representative flow plots of peripheral blood stained with B220 and CD3 of recipients transplanted with Flt3-ITD cells transduced with empty vector, IDH2 wild type, or mutants (IDH2R140Q and IDH2R172K). (F) Representative flow plots of peripheral blood stained with Mac-1 and Gr-1 of recipients transplanted with Flt3-ITD cells transduced with empty vector, IDH2 wild type, or mutants (IDH2R140Q and IDH2R172K). (G) Representative flow plots of splenocytes stained with Mac-1 and CD19 of recipients transplanted with NrasG12D cells transduced with empty vector, IDH2 wild type, or mutants (IDH2R140Q and IDH2R172K). (H) Representative flow plots of peripheral blood stained with CD19 and Thy1 of recipients transplanted with NrasG12D cells transduced with empty vector, IDH2 wild type, or mutants (IDH2R140Q and IDH2R172K). (I) Representative flow plots of peripheral blood stained with Mac-1 and c-kit of recipients transplanted with NrasG12D cells transduced with empty vector, IDH2 wild type, or mutants (IDH2R140Q and IDH2R172K).