Figure 2.

Mutant IDH2 induces CpG island hypermethylation phenotype. (A) Stacking bar graph showing percentage of hypermethylated and hypomethylated CpGs of all CpGs located in CpG islands for each chromosome, comparing IDH2 R172K mutant with wild-type (WT) cells (left) or vector with IDH2 wild-type cells (right). Green represents proportion of hypomethylated cytosines, and magenta represents hypermethylated ones. Only CpGs with a Q-value <0.01 and a methylation difference of at least 25% are shown. (B) GSEA was performed on genes that were promoter DNA-hypermethylated in IDH2 R172K mutant cells. The table shows the top four most significantly enriched gene sets from the Broad Institute database and their P-values.