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. Author manuscript; available in PMC: 2013 Oct 8.
Published in final edited form as: Cell Rep. 2013 Aug 29;4(5):959–973. doi: 10.1016/j.celrep.2013.07.043

Figure 8. Novel sites of virus-induced transcription factor recruitment result in novel innate antiviral RNA transcription.

Figure 8

(A & B) Namalwa cells were infected with (A) Sendai virus, (B) EMCV, or influenza A/Udorn/72 or mock infected for 10 hours and analyzed by ChIP (IRF3, p65/RELA, and MED1). Numbers on the x-axis refer to the nviRNA locus chromosome number, with numbers in parentheses to distinguish loci that are located on the same chromosome. Error bars denote standard deviation for triplicate PCR reactions.

(C, D & E) nviRNA induction by Sendai virus, influenza A/Udorn/72 or A/WSN/33, VSV, and EMCV infection. (C & D) Namalwa and (E) HeLa cells were mock infected or infected (5 pfu/cell), and 5 × 106 cells were harvested 10 h.p.i. for RNA isolation, and RT-PCR was performed with primers for the indicated nviRNA locus and GAPDH for normalization. Error bars denote standard deviation for triplicate PCR reactions. * indicates p≤0.05; ** indicates RNA was not detected in mock-infected cells.