Table 1.
The content of rhein in the decoction extract and antifungal activity against dermatophytes of the decoction extract and its hydrolyzed mixture containing anthraquinone aglycones of C. fistula pod pulp kept under various storage conditions of stability studies.
| Time (month) | Packaging | Storage condition | Rhein contenta
(% w/w) |
T. mentagrophytes DMST 19735 and T. mentagrophytes
MIC (µg/mL) |
T. rubrum
MIC (µg/mL) |
M. gypseum
MIC (µg/mL) |
|||
|---|---|---|---|---|---|---|---|---|---|
| Decoction extract | Hydrolyzed mixture | Decoction extract | Hydrolyzed mixture | Decoction extract | Hydrolyzed mixture | ||||
| 0 | 0.0823 ± 0.001 (100.00%)b |
>1000 | 500 | >1000 | 250 | >1000 | 500 | ||
|
| |||||||||
| 3 | Glass vial | Real time | 0.0829 ± 0.001 (100.66%) |
>1000 | 500 | >1000 | 250 | >1000 | 500 |
| Aluminum foil | Real time | 0.0815 ± 0.001 (98.96%) |
>1000 | 500 | >1000 | 250 | >1000 | 500 | |
| Glass vial | Accelerated | 0.0817 ± 0.003 (99.25%) |
>1000 | 500 | >1000 | 250 | >1000 | 500 | |
| Aluminum foil | Accelerated | 0.0809 ± 0.002 (98.23%) |
>1000 | 500 | >1000 | 250 | >1000 | 500 | |
|
| |||||||||
| 6 | Glass vial | Real time | 0.0791 ± 0.002 (96.11%) |
>1000 | 500 | >1000 | 250 | >1000 | 500 |
| Aluminum foil | Real time | 0.0794 ± 0.001 (96.46%) |
>1000 | 500 | >1000 | 250 | >1000 | 500 | |
| Glass vial | Accelerated | 0.0790 ± 0.001 (95.95%) |
>1000 | 500 | >1000 | 250 | >1000 | 500 | |
| Aluminum foil | Accelerated | 0.0788 ± 0.005 (95.69%) |
>1000 | 500 | >1000 | 250 | >1000 | 500 | |
aExpressed as mean ± SD (n = 3) and bcontent of rhein in C. fistula decoction extract calculated as 100% at day 0. Real time = 30°C ± 2°C/75% RH ± 5% RH. Accelerated = 40°C ± 2°C/75% RH ± 5% RH.