Figure 7.

The rad4-116 allele does not alter meiotic recombination. (A) Spread nuclei of S. pombe strains S964 (wild type), S1329 (rad4-116), and S1331 (rec12) prepared after 3 h of induction of the meiotic program (corresponding to meiotic prophase I; see Perez-Hidalgo et al., 2003) stained with DAPI (left) and anti-γ-H2AX antibody (right). Representative images are shown, but at least 20 nuclei from each strain were examined. (B) Frequency of intergenic meiotic recombination measured on the leu1-his5 interval on chromosome II by random spore analysis of crosses between wild type (PN22 × S1285) and rad4-116 (S1382 × S1383) strains. Crosses were performed at the permissive (25°C) and semipermissive (32°C) temperature for the rad4-116 allele.