Figure 1.

Summary of telomere profile and growth phenotypes of 63 mutants. (A) Sequence of the template cassette. The wild-type TLC1 sequence surrounding the template region is shown on top. The template region is in capitals and positions 476–474 are underlined. Mutations that create SphI and SalI sites are in capitals. The SphI and SalI sites are underlined with arrows pointing to the bases where the restriction enzymes cut. Sequence of the Rap1p consensus binding site is shown in 3′-5′ direction underneath. (B) Sequences of the oligos used to construct the template mutant library. (C) Summary of telomere profile and growth phenotypes of 63 mutants. Sequence is shown in 3′-5′ direction. Telomere length for each mutant is characterized as described in text. Cell growth for each mutant is scored as wild type or close to wild-type growth (+++), distinguishably sicker than wild type (++), very sick (+), or senescent (-). Stars indicate mutants that showed immediate slow growth but later recovered. Mutants that were recovered in the screen in Forstemann et al. (2003) are checked. Mutants that were further analyzed for their cellular phenotypes as described in RESULTS are marked by dots. (D) Growth of representative mutants was scored as described in C. Cells were scored ∼100 generations (five streaks) after the loss of the wild-type sequence. Cell were grown in liquid culture to OD₆₀₀ = 1.0 and serially diluted by 1:3. Equal volumes of the diluted cultures were spotted on the plate. The sequence and the telomere profile class for each mutant are indicated.