Figure 2.

Comparison of EphA4 WT and EphA4 Y928F mutant expressing embryos. Embryos were left uninjected or injected with RNA encoding WT or Y928F mRNA. (A) Phenotypic effect and whole mount in situ hybridization with Xpo probe (posterior marker) of embryos expressing WT or Y928F mutant protein. Arrows indicate normal or ectopic posterior expression of the Xpo marker. (B) Summary of the frequency and percentage of Xenopus embryos displaying ectopic posterior structures when injected with either WT or Y928F EphA4 (number of embryos displaying protrusions/number of embryos injected). (C) WT or mutant EphA4 receptor expression was examined by Western blot analysis of stage 9 embryonic lysates. (D) Embryos stained for β-galactosidase activity after injection with mutant EphA4 receptor RNA plus β-galactosidase RNA or β-galactosidase RNA alone as a lineage tracer. Insert is enlargement of boxed area.