Figure 3.

Phenotypes and whole mount in situ hybridization of posterior and neural crest markers in Y928F injected embryos. (A) Cell dissociation at the blastula stage and ectopic protrusion formation at the stage 37/38 tadpole are observed in Y928F expressing embryos and not in uninjected controls. The arrow indicates a representative posterior protrusion. (B) Albino embryos were left uninjected or injected with Y928F mRNA (1.25 ng mRNA was injected into one blastomere at the 2-cell stage). Embryos at stage 26/28 were fixed with MEMPFA. Whole mount in situ hybridizations were performed with posterior markers (Xwnt3a and Xhox-3) and neural crest markers (Xslug and Xap-2). Filled arrowheads indicate staining of the posterior protrusions. Double-open arrowheads indicate third and fourth branchial arches; a single-open arrowhead denotes cephalic slug expression.