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. 2004 Apr;15(4):1647–1655. doi: 10.1091/mbc.E03-09-0674

Figure 8.

Figure 8.

Inhibition of FGF-8 expression using antisense morpholino oligonucleotides represses Y928F-induced ectopic protrusion formation in vivo. (A) FGF-8-AS blocks translation of FGF-8 expression in vitro. HA-tagged FGF-8a or FGF-8b mRNAs (1 ng) were in vitro–translated in the presence or absence of FGF-8-AS (14 ng) or a control antisense reagent with 4 point mutations [FGF-8-AS-4PM] (14 ng) as indicated. Western blot analysis of the reaction products with anti-HA antibody was used to examine inhibition of FGF-8a and FGF-8b translation in the presence or absence of the indicated antisense reagent. The top and bottom arrows indicate the bands corresponding to FGF-8b and FGF-8a, respectively. (B) Antisense FGF-8 (FGF-8-AS) and (FGF-8-AS-4PM) were injected into the indicated number of Xenopus embryos with or without Y928F and scored for ectopic protrusions at stage 26.