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. 2013 Feb 18;14(4):474–481. doi: 10.1002/cbic.201200714

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Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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pH profiles of k_cat/K_m values for NAL activity of A) wild-type S. aureus NAL, and B) 165-γ-thialysine NAL. Kinetic parameters were measured for the enzymes using the standard NAL assay²²^,³⁸ at 30 °C in a three component buffer system.[39] The data are fitted to bell-shaped pH profiles.