Table 3. Kinetics and affinity constants for wild-type and mutant Tsi3 binding to Tse3.

Tsi3	Association rate k a (M −1 s−1)	Dissociation rate k d (M −1 s−1)	Binding affinity K d (M)
Wild type	1.29 × 104	3.61 × 10−4	2.81 × 10−8
R60A	Undetectable	Undetectable	—
S99A	8.99 × 103	2.96 × 10−2	3.29 × 10−6
E103A	1.43 × 104	6.25 × 10−4	4.36 × 10−8
Q124A	1.73 × 104	1.01 × 10−3	5.88 × 10−8
E126A	1.21 × 104	5.05 × 10−3	4.17 × 10−7