Figure 4.

Synthetic genetic interactions between mmd1 and a mutation in an α-tubulin gene. (A) Wild type (FY254), mmd1-1 (MYP102), ban5-4 (MYP2), and mmd1-1 ban5-4 (MYP109) were grown in liquid YES medium and diluted to 10⁶ cells/ml. Tenfold serial dilutions (10-μl aliquots) were spotted on solid YES medium and incubated at 25°C for 5 d. (B) Cells were grown in YES medium at 23°C, stained with DASPMI, and visualized with fluorescence microscopy. Bar, 2 μm.