Figure 6.

S. cerevisiae MMD1 gene complements the mitochondrial and temperature sensitive defects of mmd1-1 cells. (A) S. pombe mmd1-1 cells (MYP102) were grown in EMM medium, and mmd1-1 cells transformed with a multicopy plasmid (pURscMMD1) encoding S. cerevisiae MMD1 (MYP113) were cultured in EMM medium without uracil. Cells were grown at 23°C, incubated for 4 h at 37°C, stained with DASPMI, and analyzed by fluorescence microscopy. Bar, 2 μm. (B) Wild type and mmd1-1 transformed with the empty plasmid pUR19 (MYP219 and MYP220) as well as mmd1-1 transformed with pUR19scMMD1 were grown in liquid EMM medium without uracil and diluted to 10⁷ cells/ml. Tenfold serial dilutions (10-μl aliquots) were spotted on solid EMM-uracil medium and incubated at 25 and 37°C for 4 d.