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Hip1R siRNA duplexes reduce Hip1R expression in HeLa cells. (A) Western blots of siRNA-transfected cells using antibodies against Hip1R, Hip1, clathrin heavy chain (CL HC). (B) Western blot to quantify the reduction in Hip1R expression in A1 and A2 cells. In lanes 4–7 different amounts of extracts were loaded (10–100%) as indicated in the figure. (C) Hip1R immunofluoresence of cells treated with invA2, A1, or A2 (top panels). Bottom: DAPI staining of nuclei. Bars, 10 μm.
