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. 2004 Apr;15(4):1666–1679. doi: 10.1091/mbc.E03-09-0639

Figure 7.

Figure 7.

The F-actin structures that accumulate in the absence of Hip1R contain rapidly polymerizing actin. (A) Time lapse videomicroscopy of invA2- or A1-treated HeLa cells expressing GFP-actin. The F-actin structures in A1 cells were observed at 3-s intervals. Top panel shows low magnification views of the cells. Bars, 10 μm. Bottom panel is enlargements of boxes in the top panel showing the first frame of movie 1 and movie 2. Bars, 2.5 μm. (B) FRAP analysis of A1-treated HeLa cells expressing GFP-actin. Top (FRAP 1): analysis of small spots/tail-like F-actin structures; bottom (FRAP 2): analysis of a larger ring-like structures. We observed the F-actin structures in A1 cells at 3-s intervals >40 frames. The cells were photobleached in a defined area after two frames as indicated in the figure (see asterisk). The graph shows fluorescence intensity (arbitrary units) as a function of time (s), where the data have been normalized against a reference region that was not photobleached. Bars, 10 μm.