Table 1. Representative parameters from the crystallographic data measurement of GanB-WT and its catalytic mutant GanB-E323A.
Values in parentheses are for the last resolution shell.
| Protein | GanB-E323A | GanB-WT |
|---|---|---|
| Beamline | I04, Diamond | ID14-1, ESRF |
| Wavelength (Å) | 0.9763 | 0.9334 |
| Space group | P212121 | P212121 |
| Unit-cell parameters (Å, °) | a = 71.73, b = 181.31, c = 197.66, α = β = γ = 90.00 | a = 71.84, b = 181.35, c = 196.57, α = β = γ = 90.00 |
| No. of reflections (total/unique) | 285572/87146 | 531503/91957 |
| Multiplicity | 3.3 | 5.8 |
| 〈I/σ(I)〉 | 13.9 | 11.7 |
| Mosaicity (°) | 0.58 | 0.71 |
| Resolution (Å) | 71.7–2.50 (2.56–2.50) | 48.9–2.45 (2.58–2.45) |
| Completeness (%) | 97.4 (96.6) | 96.5 (88.6) |
| R merge † (%) | 6.1 (19.8) | 11.3 (34.8) |
†
R
merge = 
, where I
i(hkl) is an individual intensity measurement and 〈I(hkl)〉 is the average intensity for this reflection, with summation over all data.