Figure 11.

Vti1b association with Syn6t and Syn6 in INS cells. The cell lines indicated were immunoprecipitated with anti-myc mAb or anti-myc polyclonal antibody, which were then subjected to SDS-PAGE and immunoblotting. All three lower panels show immunoblotting with anti-Vti1b; note that from control INS39 cells no Vti1b can be coprecipitated with anti-myc (left and middle) although Vti1b can be coprecipitated when the cells are immunoprecipitated with anti-Syn6 (bottom right). The upper panels all show myc Western blotting with polyclonal anti-myc to confirm direct immunoprecipitation of the myc-tagged Syn6 full-length and Syn6t constructs as well as the presence of these bands in the original cell lysates (top right).