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. 2013 Jun 13;22(21):4253–4266. doi: 10.1093/hmg/ddt276

Figure 1.

Figure 1.

PrP-α3M>S mutants show incomplete maturation. (A–C) Tissue homogenates from flies expressing PrP-WT, -M205S or -M205,212S untreated (A) or incubated with PNGase F (B) or Endo H (C) and detected by 6D11 in western blot. (A) Both α3M>S mutants display a higher molecular weight than PrP-WT. (B) Complete deglycosylation with PNGase F produces a single band of unglycosylated PrP-WT at around 27 kDa. Both PrP-M205S and PrP-M205,212S exhibit a slightly higher molecular weight. (C) Treatment with Endo H has no effect on PrP-WT and -M205, but decreases the size of PrP-M205,212S.