Table 2.
Plasmids used in this study
| Name | Descriptiona | Source |
|---|---|---|
| pEG202-NLS | 2μ, HIS3, LexA DBD | Origene Technologies |
| pJG4-5 | 2μ, TRP1, Transcriptional AD | Ausubel et al. (1995) |
| pET21b | T7, His6 | Promega |
| pGEX-KG | GST fuson expression vector | Guan and Dixon (1991) |
| pRS305 | LEU2 | Sikorski and Hieter (1989) |
| pRS306 | URA3 | Sikorski and Hieter (1989) |
| pRS313 | CEN, HIS3 | Sikorski and Hieter (1989) |
| YCplac111 | CEN, LEU2 | Gietz and Sugino (1988) |
| YCplac22 | CEN, TRP1 | Gietz and Sugino (1988) |
| YCplac33 | CEN, URA3 | Gietz and Sugino (1988) |
| pSK910 | URA1, GAL1-2xEGFP-CDC5ΔDB | This study |
| pSK1405 | pEG202-NLS, CDC5ΔDB | This study |
| pSK1408 | pEG202-NLS, CDC5ΔDBΔC | This study |
| pSK1390 | pEG202-NLS, CDC5ΔN | This study |
| pSK1403 | pEG202-NLS, CDC5ΔN/FAA | This study |
| pSK1883 | pJG4-5, BBP1(1-385) | This study |
| pSK1882 | pJG4-5, BBP1(1-295) | This study |
| pSK1884 | pJG4-5, BBP1(243-385) | This study |
| pSK1901 | pEG202-NLS, BBP1(1-385) | This study |
| pSK1900 | pEG202-NLS, BBP1(1-295) | This study |
| pSK1902 | pEG202-NLS, BBP1(243-385) | This study |
| pKL2497 | pGEX-KG, BBP1(1-385) | This study |
| pKL2498 | pGEX-KG, BBP1(1-295) | This study |
| pKL2500 | pGEX-KG, BBP1(1-243) | This study |
| pKL2499 | pGEX-KG, BBP1(243-385) | This study |
| pKL2501 | pGEX-KG, BBP1 (295-385) | This study |
| pKL2496 | pGEX-KG, MPS2 | This study |
| pKL1053 | pET21b, CDC5 | This study |
| pSK1868 | YCplac111, GAL10-FLAG-YFP-BBP1(1-385) | This study |
| pSK1867 | YCplac111, GAL10-FLAG-YFP-BBP1(1-295) | This study |
| pSK1870 | YCplac111, GAL10-FLAG-YFP-BBP1(1-243) | This study |
| pSK1869 | YCplac111, GAL10-FLAG-YFP-BBP1(243-385) | This study |
| pSK1871 | YCplac111, GAL10-FLAG-YFP-BBP1(295-385) | This study |
| pSK1873 | YCplac22, GAL1-HA-GST-BBP1(1-385) | This study |
| pSK1874 | YCplac22, GAL1-HA-GST-BBP1(243-385) | This study |
| pSK1875 | YCplac22, GAL1-HA-GST-BBP1(1-243) | This study |
| pSK1878 | YCplac22, BBP1(1-385) | This study |
| pSK1877 | YCplac22, BBP1(1-295) | This study |
| pSK1880 | YCplac22, BBP1(1-243) | This study |
| pSK1879 | YCplac22, BBP1(243-385) | This study |
| pSK1881 | YCplac22, BBP1(295-385) | This study |
| pSK1910 | ADE2, GAL1-HA-GST-BBP1(1-385) | This study |
| pSK1909 | ADE2, GAL1-HA-GST-BBP1(1-295) | This study |
| pSK1912 | ADE2, GAL1-HA-GST-BBP1(1-243) | This study |
| pSK1911 | ADE2, GAL1-HA-GST-BBP1(243-385) | This study |
| pSK1913 | ADE2, GAL1-HA-GST-BBP1(295-385) | This study |
| pCJ159 | pRS306, SPC42-GFP | This study |
| pCJ204 | pRS305, SPC42-GFP | This study |
| pCJ259 | pUC19-URA3, CDC14-5xGFP | This study |
| pKL2701 | YCplac111, EGFP-CDC5 | This study |
| pKL2078 | YCplac111, YFP-CDC5ΔC-BBP1243-385 | This study |
| pKL2422 | pRS315, YFP-CDC5ΔC-MPS2 | This study |
| pCJ232 | YCplac111, YFP-CDC5ΔC | This study |
| pKL2420 | pRS315, YFP-CDC5ΔC-BBP1 | This study |
| pCJ107 | pRS313, MPS2 | This study |
| pCJ238 | YCplac33-GAL1, ΔCEN4 | This study |
| pCJ241 | pCJ238, GAL1-CDC5 | This study |
| pCJ240 | pCJ238, GAL1-CDC5ΔC-BBP1243-385 | This study |
| pCJ242 | pCJ238, GAL1-CDC5ΔC | This study |
| pKL2071 | YCplac111, GAL10-YFP-CDC5ΔC-BBP1243-385 | This study |
| pCJ231 | YCplac111, GAL10-YFP-CDC5ΔC | This study |
a
2μ indicates high-copy plasmids; CEN indicates low-copy plasmids