Figure 1.

116HG and 115HG are developmentally regulated and form overlapping but distinct nuclear RNA clouds. (A) Schematic representation of the murine PWS locus. RNA FISH probes (red) and the DNA FISH probe (green) are shown. (B) RNA FISH for 115HG (green) and 116HG (red) on adult WT mouse cortex. 4′,6-diamidino-2-phenylindole (blue), scale bar is 1 µm. (C) Diameters of RNA cloud signals on individual cortical sections from E19, P2, P5 and adult mice. (D) Co-localization of 116HG and 115HG clouds. (E) Combined DNA and RNA FISH to adult mouse cortical tissue revealed partial co-localization of the 116HG lncRNA (red) with the paternal decondensed allele of Snrpn through Ube3a (green). (F) Combined DNA and RNA FISH on human cerebellum from a neurotypical female using human-specific RNA and DNA probes (see also Supplementary Material, Fig. S1).