Figure 4.

(A) Assessing cohesion at a CEN locus 1.8 kb from CEN15: CHL1, VIK1, CSM3, BIM1, TOF1, and KAR3 were deleted in strain YPH1444, which expresses a Lac repressor-GFP fusion protein and contains a Lac operator repeat integrated 1.8 kb from the centromere of chromosome XV. The number of GFP signals in each cell was scored for two independent strains for each deletion in two independent experiments. At least 200 cells were scored for each strain. (B) Sister chromatid cohesion quantified. The average percentage of nocodazole-arrested cells with two GFP signals from two strains in two independent experiments is given along with the SD.