Fig. 1.
Production of 3F8BiAb. The 3F8BiAb was generated following chemical heteroconjugation of OKT3 and 3F8 mAb's as described in materials and methods. The product was resolved by SDS– non-reducing polyacrylamide gradient (4–20%) gel electrophoresis and detected by Coomassie blue staining. Lane 1, 8 μg of 3F8 mAb; lane 2, 8 μg of OKT3 mAb; lane 3, no sample; lane 4, 8 μg of 3F8 BiAb. The unconjugated OKT3 and 3F8 (monomers), conjugated 3F8 BiAb dimers and multimers are indicated in the figure.