Fig. 3.

Binding of 3F8BiAb to ATC and target cells. A: Binding of 3F8BiAb to ATC. 1 ×10⁶ ATC were armed with 1 μg each of 3F8mAb and 3F8BiAb and the amount of antibody bound to the surface of the cells was measured by flow cytometry as described in materials and methods. Solid gray histograms represent cells that have OKT3 bound to their surface via 3F8 (3F8BiAb). Percentage positive cells shown in parenthesis were obtained by setting the markers using appropriate isotype control antibodies (open dotted lines). B: Binding of 3F8BiAb to tumor cells. LAN-6 (10⁶ cells) and KCNR (10⁶ cells) were incubated with 1 μg of 3F8BiAb and analyzed by flow cytometry. The solid grey histograms show the binding of 3F8BiAb to the target cells. Matched isotype controls were used to quantitate the positive cells shown in parenthesis.