Fig. 1.

Co-culture of PBMC exposed to SK-BR-3 cells with autologous *ATC or armed *ATC with one repeated antigenic stimulation-induced specific antibody responses. a Shows the kinetics of the development of in vitro antibody by PBMC in cultures from 9 healthy donors at day 6, 10, and 14 under the indicated co-culture conditions. The total mean amount of anti-SK-BR-3 antibodies reported as SK-BR-3 specific antibodies in ng/ml/1 × 10⁶ cells cultured. All values have been corrected for background (<5 ng/ml); b shows the range of in vitro antibody synthesis in 9 normal subjects at day 14; c shows the proportions of IgG₁ and IgG₂ antibody subtypes (n = 9); and d shows the effect of unirradiated ATC or aATC on primary antibody synthesis (n = 3)