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Progressive delocalization of the spindle pole body protein sad1p upon induction of vpr expression. Cells bearing pREP1 or pREP-VPR were grown under inducing conditions for 17, 19, 22, or 24 h. Cells were harvested, fixed, and examined by immunofluorescence for Sad1p, and imaged in three-dimensional projections; 78-200 cells were examined for each time point.
