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BFA-induced redistribution of ARF1 and β-COP is not inhibited by ITD. Clone 9 cells were kept in MEM and treated with solvents only (Control), BFA alone (5 μg/ml) for 5 min (+ BFA), or pretreated with ITD (25 μM for 5 min) before addition of BFA (+ ITD → + BFA), as indicated. Staining is as follows: ARF1 and ManII double-immunofluorescence (A-I); β-COP and ManII double-immunofluorescence (J-R).
