Figure 2.

Western blot analyses of c-Fos induction in PC12 cells cultured under different experimental conditions. PC12 cells were treated with the indicated effectors and harvested at the indicated days. For each condition, 60 μg of homogenate protein was subjected to SDS-PAGE and Western blotting with anti-c-Fos polyclonal antibody (top row) or with anti-α-Tub antibody (bottom row) to control protein loading. Results are from one representative experiment of three performed.