Figure 4.

Increasing c-Fos levels by inhibiting its degradation with A-Calp increases neuritogenesis. PC12 cells cultured 4 d with NGF (top row) or NGF + A-Calp (middle row) or NGF + A-Calp + ASO (bottom row), as indicated at left, were double immunostained for c-Fos (left) and for α-Tub (right). Double blind morphometric quantifications of each experimental condition are posted on the right of the corresponding row. Bar, 10 μm. ^*, neurites in cells treated with NGF + A-Calp (middle row) were significantly longer than NGF-treated (top row) cells at p < 0.000001 (by ANOVA test as in Figure 1).