Figure 4.

The LIM domain containing C-terminus of Pxl1p is sufficient to target the protein to sites of polarized growth. (A-C) pxl1Δ cells carrying the plasmid GFP::pxl1-503-706 that lacks the preLIM domain of Pxl1p were examined at different stages of the cell cycle for the distribution of the fusion protein. GFP::pxl1-503-706 localizes to the incipient bud site (A), the growing bud tip (B), and the mother-bud neck (C), in a manner identical to the localization pattern of the full-length GFP::Pxl1p fusion protein.