Table 2.
Plasmids used in this study
| Plasmid | Contents | Source |
|---|---|---|
| BSE 19 | pRS315 | Sikorski and Hieter (1989) |
| BSE 119 | pRS425 | Christianson et al. (1992) |
| BSE 345 | PXL1 gene in pRS315: −1000/+500, LEU-based plasmid, cloned Xho1-Sac1 | |
| BSE 346 | PXL1 gene in 2 micron vector pRS425; −1000/+500, LEU-based plasmid, cloned Xho1-Sac1 | |
| BSE 352 | pRS315 containing GFP::PXL1 and −1000 bp of upstream and +500 bp of downstream PXL1 | |
| BSE 364 | GFP-pxl1-LIM only clone, constructed by removing PXL1 coding sequences for a.a. 1-502 from BSE352 | |
| BSE 365 | GFP-pxl1-N terminus clone, constructed by inserting a nonsense codon into PXL1 in BSE352 | |
| BSE 378 | pxl1-lim1; PXL1-H581l/C584A site-directed mutagenesis clone of LIM domain 1 substituted in BSE345 | |
| BSE 379 | pxl1-lim2; PXL1-H642l/C645A site-directed mutagenesis clone of LIM domain 2 substituted in BSE345 | |
| BSE 385 | GFP-Pxl1-H581l/C584A site-directed mutagenesis clone substituted in BSE352 | |
| BSE 386 | GFP-Pxl1-H642l/C645A site-directed mutagenesis clone substituted in BSE352 |
All plasmids were constructed for this study unless otherwise indicated.